Molecular Identification of Prevalent Streptococcus Pyogenes Serogroup Associated with Respiratory Tract Infections in Children

The current study intends to characterize the respiratory tract infections that have an association with gram positive bacterial pathogens, emm typing, pattern of antibacterial resistance in isolated phenotyping virulence factor and molecular detection Macrolide gene. Various samples from patients were collected identification antimicrobial susceptibility clinical isolates was performed as per standard laboratory procedure. Macrolides (Eruthromycin, Clindamycin)-resistant again subjected MIC method. Macrolide-inducible clindamycin (D test) conducted upon Erythromycin Clindamycin discs. Multiplex PCR used detect genes all three types macrolide strains. Serum Opacity Factor (SOF) detected for GAS. Every isolate checked produce biofilm through micro titre plate Bacterial growth got registered 156 (36.28%) samples. most common URI GAS i.e., 64 (14.9%), only be followed by GGS 38(8.8%), GCS 29 (6.7%) Staphylococcus aureus 18 (4.2%). Among GAS, one recorded blood, whereas 8.50% sputum rest. All 71 found exhibit sensitivity towards Penicillin Ceftriazone. exhibited 55% Mtype 40% resistant cMLS 5% iMLS. showcased equal number M type too. portrayed 54.54% 36.36% 9.09% iMLS least resistance. identified Streptococcus pyogenes is bacteria cause recur infection. prevalence tends change geographically periodically. For this purpose achieve a sound public health outcome, periodical screening antibiotic-resistance becomes inevitable.